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X-Light V1

X-Light V1

Detail Description




Crest Optics X-Light marks an evolution in Nipkow spinning disk confocal scanner with unique aberration free optical relay system from light source condenser to microscope tube lens alignment as well as detector collection optics. Coupling with modern high intensity multi-wavelength solid state illumination sources and high resolution camera, X-Light enables high resolution confocal imaging in an easy to use and cost effective optical package that fits even on your existing microscope.

Benefits in X-Light V1 Scanner

1. Excellent image quality from expert optical integration system:

•flexible to allow the best alignment with light source to microscope and camera

•diffraction limit relay lens supports 22mm FOV with large format sCOMS

•camera and spinning disk in conjugated image plane for maximum collection efficiency and easy alignment

•optical limit resolution confocal performance

2. Excellent confocal effect from innovated spinning disk pattern and artefacts free:

•multi-spiral pinhole pattern ensures high fill factor and low pinhole cross-talk

•Non-isotropic pinhole arrangement allows higher spacing between pinholes and minimizes pinhole crosstalk from neighboring pinholes more efficient in covering the entire field of view rapidly in order of hundreds of microseconds to achieve 24 frames per rotation breakthrough from traditional 12 frames per rotation

•by-pass mode between wide field and confocal

•two pattern pinhole spinning disk matching with different experimental requirements

•pinhole disk pattern matching with EMCCD &sCMOS camera and objective numerical aperture

•free of tailing artefacts in continuous 15,000RPM spinning under confocal mode

3. Continuous expansion flexibility to cope with existing requirements and future upgrade path:

•seamlessly integrate with existing microscope, even non-fluorescent manual microscope for multi-color 3D imaging

•choices of different high power long life light sources, high power LED (up to 310mW/λ) or high power laser (up to 1W/λ in 1000:1 linear dynamic range)

•various software control package in continuous upgrade

•multi-cameras or camera image split options at any time

•choice of spectral range:

- X-Light V1 “VIS”: Standard system operating in the visible range 385nm-750nm

- X-Light V1 “VIS-NIR”: Standard system optimized in the range 385nm-1000nm

- X-Light V1 “UV-VIS”: System optimized in the UV-VIS range 350nm-750nm

- X-Light V1 “NIR”: System optimized for the near infrared fluorescent probes operating in the range 700nm-1600nm
4. Reproducible high quality performance at all time from trouble free long term stability alignment

•alignment easy to adjust upon installation and stable for long time from the effective and neat optical design

•trouble free maintenance and operation

 

Top class spinning disk scanner ideal for living cell prolonged time lapse fluorescent imaging to monitor rapidly occurring events within living cells without compromising resolution, as well as the high frequency low intensity signals because of substantially reduced photobleaching and phototoxicity. Perfect for investigation of multicolor colocalization, 3D optical sectioning & reconstruction and Multi-dimension imaging (X, Y, Z, I, λ, t, n).

 

Specimen fluorescent staining: fluorescent dye staining, immuno-fluorescence assay, fluorescent in-situ hybridization, fluorescent protein, microinjection of fluorescent labeled actin probes into living cells.

 

Application areas:

•Cell Biology & Plant Biology: apoptosis, autophagy, cell cycle, cell metabolism, cell tracking and tracing, cytotoxicity, oxidative stress detection, phagocytosis, endocytosis, receptor internalization, cell signaling, & communication, cell motility, cellular compartmentalization, protein synthesis and degradation, cellular and biophysical regulations,

•Cell and System Dynamics: structures and organs, e.g. blood vessels, neurons and processes such as angiogenesis and immune responses to vessel lesions

•Embryology & Developmental biology: C. elegans, Drosophila and Zebrafish growth and signal mechanisms

•Cancer research

•Clinical & translational medicine research

•Cardio and neuro sciences

•Calcium imaging, other ion measurements & membrane potential

•Yeast and bacteria studies

•Stem cell research and 3D cultures

•Synthetic biology: biofuels, vaccine & antibody production, plant sciences, industrial enzymes, biobased chemicals

•Pharma, biopharma & CRO


Crest Optics X-Light V1 Specification

Light source

Flat and even illumination by LED (3 to 7 wavelengths) or Laser (3 to 6 modules or 6 or 7wavelengths combination) with superb rejection of out of focus light

Illumination source supports hardware triggering for fast multichannel experiment

Supported input fiber

 0.39NA multimode 1.5mm fiber with SMA adapter; excitation Gimbal mount for easy alignment on custom microscope setup and for best S/N

Acquisition modes

Widefield – disk out, capture bright field   phase contrast or DIC overlay with confocal image or standalone in all wide field fluorescent & bright field imaging

Confocal – disk in

Disk pinhole size

vs camera size

Double pattern pinholes  at 12mmx12mm FOV each pattern for CCD

Single pattern pinholes at 16.8mmx14mm FOV for large format sCMOS

Option: custom pattern

Disk speed

15,000 RPM=6,000fps standard (optional 20,000 RPM=8,000fps)

Laser clean up filter

Option 3-position manual slider

Dichroic wheel

Motorized 3-position standard (option   5-position)

Dichroic size

25.5 mm x 36 mm x 1 mm

Emission filter wheel

Manual 4-position wheel standard   (option automated 8-position wheel)

Emission filter size

Φ 25 mm

Supported microscope

Upright and inverted microscope models from all brands with 100% c-mount output port

With apochromatic corrected objective

Camera

High sensitivity CCD, sCMOS and EMCCD in c-mount port

Easy camera focus through internal optics without moving camera, no other disc and camera alignment needed

Software control

MetaMorph, NIS Elements, Micro-Manager