The ATUMtome tape collecting ultramicrotome is now available for high resolution array tomography of biological tissues. Thousands of utra-thin sections with a thickness of 30nm can be automatically collected on 8mm wide Kapton tape for SEM imaging and sebsequent large tissue and whole organ 3-D reconstruction. The ATUMtome’s tape collecting part of the system (ATUM) is under license from Harvard University where Professor Jeff Lichtman, PhD, MD, and his team designed the instrument to help collect ultrathin sections to help reconstruct the brain’s neural pathways — a precursor to one day mapping the entire human brain.
Features & Benefits:
>Collects hundreds to thousands of sections on a continuous tape
>Non-destructive to sample, with sections available for years into the future for processing, post-staining, immunogold labeling, correlative imaging — and at any work pace desired.
>Section thickness usually range from 30nm to 5,000nm, with thinner sections possible.
>Determines sample viability sooner in the process so you don’t waste valuable time cutting and imaging a problem sample.
>Uses multiple resolutions for locating regions of interest, then zooming in for higher resolution imaging
>Allows correlative microscopy for localization and then ultra-structural imaging
>Prepares samples that allow short pixel dwell time for fast detection of images during electron microscopy
>Uses standard sample preparation techniques and resins
>Charging effect on sections is manageable
>Priced at a fraction of the cost of alternative 3-D imaging techniques
Supplied Complete with:
>ATUM continuous tape feed mechanism with PC control software
>Air-activated anti-vibration microtomy table including ATUM attachment interface with x-y-z fine control positioning of tape/section pick-up head
>Ergonomic lab chair
>4 mm diamond knife, 35 degrees for room temperature ultra-thin sectioning, mounted in large-cavity blue anodized holder
>Water level control system
>Start-up supply of Kapton tape
>Four 4” diameter silicon wafers
>Dimensions: 49” W x 36” D x 54” H
>Weight: 796 lbs. ( 361 kg)
ASH2 Advance Substrate holder: for collection hundreds of serial section in small tissue or organelle 3D reconstruction
>Quickly locks onto most ultramicrotomes, providing a serial section collection system for SEM and LM 3D reconstruction
>Holds the substrate of your choice, from standard glass slides to various sized conductive silicon wafer substrates, Indium tin oxide (ITO) coated glass slide
>Transfer sections directly to the microscope for fast, efficient imaging
>Capable of handling hundreds of sections on one substrate
>ASH2 can be use with a variety of standard diamond knives