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Capillary Cell Sorting

Capillary Cell Sorting

Detail Description

MMI CellEctor:A microscope based micro capillary single cell sorting system for rapid recognition, acquisition and deposition of single, stem cells and CTCs or rare cells in suspension into reaction slides or directly into PCR tubes, IBIDI style chambers or microfluidic devices. The system is fully motorized and computer controlled. It enables the development of user controlled protocols for aspirating and depositing cells in nanoliter volumes for the isolation of single cells in a format suitable for downstream molecular analysis with nanolitre volume reaction mixtures. User defined liquid handling programs enable the development of dedicated cleaning and service cycles for capillary cleaning and maintenance. It is highly modular and can be mounted on numerous microscope brands from entry level, mid-range to high end inverted microscope.

1. Cell selection and recognition

Place an aliquot of the sample of cells in suspension directly onto a standard glass slide. Visualise the cells in either bright field or fluorescence. Mark the cell of interest by clicking with the mouse.

Autodetection can be achieved with the MMI CellExplorer Software designed for the auto recognition of single cells based on their colour, morphology and relative size.

2. Ultra precise cell aspiration

A glass capillary is used to aspirate the cells of interest directly. This is controlled by a high precision 3D robotic arm and ultra precise cell pump. Both can be used in full manual mode, or semi or fully automatically. More adherent cells can be collected by reversing the position of the capillary and lowering it by a few microns to allow the cells to be released by scratching and pumping simultaneously.

3. Automated cell acquistion and deposition with full visual control

Single cells in suspension can be collected manually or automatically onto a wide range of slide based platforms including IBIDI, a PCR tube, a microfluidic or other molecular analysis device. The user gets full optical control throughout the whole workflow.

4. Molecular downstream analysis

For example: FISH, IHC, ISH, immuno fluorescence, Single Cell PCR


Features & Benefits:

•Microscope based software controlled movements of the capillary

•Easy to achieve accurate and precise cell isolation and deposition

•No contamination from unwanted cells

•Faster and easier than manual systems

•Nanoliter pump allows isolation of cells in small volumes

•Brightfield or fluorescence automated detection of cells

•Full process control of cell identification, acquisition and deposition

•Work manually or automatically as your workflow demands

•Compatibility with a wide range of accessories and microfluidic devices

Compatibility:

•Nikon Ti-S, Ti-E, Ni-U, Ni-E, A-1

•Olympus IX53, IX73, IX83, FV1000

•Zeiss Axio Observer, LSM 780

•Others on request

Expandability:

•MMI CellExplorer Software for automatic cell recognition in fluorescence or bright field

•MMI CellCut laser microdissection system

•MMI CellManipulator optical tweezers

•Range of imaging configurations (Fluorescence, DIC, Phase contrast)

•Service contracts, trainings, upgrades


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