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Laser Microdissection

Laser Microdissection

Detail Description

MMI CellCut:The ideal tool for the quick and precise detection and isolation of single cells or group of cells from variety of sample types including fresh frozen, paraffin embedded, archived slides, cytospins, smears and living cell cultures for downstream molecular biology analysis or diagnostic purposes. It enables cell selection quickly and easily directly on the touch screen. The cells of interest are marked and cut automatically using a precisely focused UV-Laser. The microdissected samples are collected for downstream analysis. This results in a pure cell population for reliable analysis and diagnosis. It is highly modular and can be mounted on numerous brands of microscope from entry level, mid-range to high end.

1. Sample preparation

The section is placed on the MMI MembraneSlide, a frame slide covered with a thin membrane that is inert and has negligible auto fluorescence. Afterwards the MMI MembraneSlide is inverted and placed onto a glass slide for protection against contamination. Now the sample is sandwiched between the membrane and the glass.

2. Easy cell selection

The cells of interest can be selected on the display using either the mouse, by freehand or predefined geometrical shapes which can be modified. Any number of cells across the slide can be identified as targets within one screening process. The stage is moving to trace the path drawn and the laser is fixed and focused from below.

3. Automated cutting

The thin laser cutting path enables a precise and gentle extraction of the selected cell at an outstanding speed. The isolated target cell is collected by lowering and lifting of the adhesive cap held from above. The sample morphology remains 100% intact.

4. Selected target

After cutting the sample can be visualised on the cap. Lysis buffer is added and the tube inverted for approx. 10 mins. The cells are now in suspension ready for downstream processing.

Features & Benefits:

•Contamination free cutting: sample is sandwiched between a membrane slide and a glass slide and so it is not exposed to the air

•Maximum sample collection: unique MMI CapLift technology enables highest sample collection efficiency and minimal sample damage. The yield can be verified during and after isolation.

•Contamination free collection: MMI IsolationCap is only in contact with the membrane and never in direct contact with the tissue.

•Positive sample inspection: isolation of the target area occurs in the same relative position as on the slide. The morphology of the sample remains 100% intact and damages of the surrounding tissue are prevented. Reproducible results are achieved easily and effectively. This is excellent for auditability and accurate quantitative results.

•The thinnest cleanest cut: uses fixed UV-laser with higher pulse rate and lower power. The result is a sharper and cleaner ultra precise cutting line of 0.3 microns and least sample damage.

•Predefined Target Positioning (PTP)

PTP is a patented product feature that allows for the precise, predefined, contamination free visually controlled collection of individual cells on the cap. PTP is excellent to maximize the number of samples collected into a single reaction tube. It guarantees that the isolated samples are not compromised by subsequent cutting which is essential for auditability.

•Autodocumentation: allows full verification and traceability of every cut. The autodocumentation feature records images showing the position of the cut and of the collection (PTP) on the cap together with the time, date and method details.

•MMI LiveCell Chamber: low power laser allows an gentle cut. In specially designed MMI LiveCell Chamber even live cells can be microdissected, for unrivalled success in re-culturing and cloning. Protocol live cell dissection

•MMI MultiCap and MMI MultiSlide: allows for the isolation of cells from up to three slides and the MMI MultiCap for the isolation of cells into up to eight individual caps without the need to reload. This is essential for diagnostic workflows involving RNA isolation, where speed is of the essence.

•Serial section: maximises the RNA recovery by minimising the use of staining. The visible position of target cells is used on a stained section to detect, mark, cut and isolate regions of interest on an unstained section.

•Z-drill: thick and wet tissue can be cut without using a high power laser. Refocus the laser on different z-levels whilst cutting to minimise the use of excessive laser power and so preserve your sample and maximise its usability for downstream analysis. An optional high power laser is available for working with harder to cut material such as teeth, bones, or forensic tapes.

Compatibility:

•Nikon Ti-S, Ti-E, Ni-U, Ni-E, A-1

•Olympus IX53, IX73, IX83, FV1000 

•Zeiss Axio Observer, LSM 780

•Others on request

Expandability:

•High power laser for microdissection of harder material, e. g. plants, bone (marrow), teeth, forensic tapes

•Digital remote laser microdissection

•MMI CellEctor for single cell sorting 

•MMI CellManipulator optical tweezers 

•Range of imaging configurations (Fluorescence, DIC, Phase contrast)

•Others on request