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SLR digital camera & adapter

SLR digital camera & adapter

Detail Description

Mounting of Canon®, Sony® and Nikon® DSLR cameras to microscopes of following brands:

•Leica®

•ZEISS®

•Nikon®

•Olympus®

•Meiji®

•Motic®

•Unitron®

•Leitz®

•Reichert®

•Labomed®

•Huvitz®

•Wild®

•American Optical®


Features of the Canon EOS DSLR as a good choice for microscope photography:

*The EOS Utility software (Win/Mac) is included at no extra charge with each EOS camera body.

*The EOS Utility software provides USB2 live preview at up to 1275×846 pixels.

*The live preview has a frame rate of at least 15 frames per second.

*The white balance can be manually adjusted within the software by means of a standard eyedropper tool, and custom white balance settings can be saved.

*The EOS Utility software can be “Linked” to other imaging software to automatically open the captured image within the Linked application (such as Photoshop or i-Solution).

*Auto exposure functions normally with our SLR adapters (some DSLR cameras do not).

*The shutter can be triggered directly from EOS Utility software and the image will be saved in a user designated folder.

*In Live View mode, the image is captured prior to the shutter closing and reopening which eliminates shutter vibration. It can eliminate the need for a mechanical first shutter curtain by using “a unique high-speed scanning and electronic reset system that accurately mimics the high-speed mechanical shutter operation. It synchronizes with the mechanical 2nd-curtain shutter to obtain a slit exposure.”  In addition, the way live-view has been incorporated in these camera bodies the exposure can be initiated while in “live-view” without any mirror motion. As a result, an exposure can begin with absolutely no mechanical movement at all and thus no vibration. The exposure is concluded by the closing of the mechanical second shutter curtain. After the exposure there will be some mechanical movement in the camera but this obviously will not affect the exposure (with the possible exception of a continuous “burst” of successive pictures).

*Canon documented this feature with the 40D, 50D, 5DII, and 7D.  They promoted it as “silent-mode”. Although not documented by Canon, this same feature (electronic first shutter curtain) is also found in the Rebel XSi/450D, Rebel XS/1000D, and Rebel T1i/500D. With these camera’s it is always “on” and there is no “silent-mode” to enable or disable.

*It was found an incredible difference in image quality between images taken using Canon Live View and images taken with the same camera without using Live View. Shutter vibration does make a visible difference, as per image comparison confirms.

Clearly the upper left frame is the worst in each group. The combination of mirror action and mechanical focal plane shutter operation causes enough vibration to seriously degrade the image quality.

The upper right is clearly the best in each group. This was the image taken with “Live View” active, and the “silent mode” turned on. So for this image the Canon 50D camera used an “electronic first shutter curtain”.

The lower left image in each set was taken using the mirror-lockup feature of the camera. The mirror was first “locked up”, and after a pause of a couple seconds the shutter was released. These were the second best image in each comparison, but significantly lower quality than with the “electronic first shutter curtain”. It is interesting to compare this image with the one in the upper right corner. This gives some indication of the degree to which a mechanical first shutter curtain can cause vibration problems.

The lower right image was taken with “Live View” active, but the “silent mode” was disabled. This means that before the exposure starts the mechanical first shutter curtain first closes, and then reopens to start the exposure. (Note that with the Rebel XSi/450D, Rebel XS/1000D, and Rebel T1i/500D there is no way to “disable” the electronic first shutter curtain). These results are interesting. The result is clearly better than the upper left image. But it is slightly worse than the one taken using the only the mirror lock-up. This is really not that surprising, since there are two mechanical actions occurring that can cause vibration ... the mechanical first shutter curtain must close and then re-open. With mirror lock-up there is only one mechanical action that can cause vibration... the first shutter curtain opens to begin the exposure.

These were all taken at 1/30 second. So far the tested shutter speeds is in the range of ½ to 1/250 second. In each case the images made using the electronic first shutter curtain were superior. At this time we can only looking at usage with continuous illumination. Electronic flash is sometimes used to “freeze” the motion of live subjects, and it is also an excellent method of avoiding vibration difficulties. But it can be cumbersome to set up, and many microscopists do not need it for their subjects. For continuous light, one of these EOS bodies could avoid the need to mount the camera on a separate stand or incorporate electronic flash.

 

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