Current Location :Home > Products
Cell Isolation Tissue Microdissection

Cell Isolation Tissue Microdissection

Detail Description

Your problem: tissue heterogeneity poses a significant obstacle for the retrieval of cell and region specific information to sustain accurate downstream analysis.


Our solution for cell isolation and tissue microdissection in both fluorescently labeled and morphologically distinct cells to be acquired from any cell cultures or tissue sections from nearly all types of adherent, suspended and 3D cultures, including difficult samples such as primary cells, or individual cells from fresh frozen and sucrose treated complex tissues, such as brain:

1. Single Cell Collection & fluorescence-based cell collection:

• Collect cells based on fluorescence or in bright field

• Collect low-abundance labeled cells

• Enrich cell lines with low transfection rates using GFP

• For downstream single cell analysis, such as NGS, transcriptome sequencing and Omics applications

2. Transformant selection & enrichment, clonal expansion:

• Collecting individual cells directly from cell cultures or recultivation purpose

• Selectively subculture transfected cells

• Maintain high cell viability

• Maximize efficiency for difficult to transfect cells

• Isolate subpopulations from complex mixtures

• Achieve high quality separation

3. Tissue microdissection:

• Microdissect sections from 5 to 300 μm thick

• Provide minimally invasive environment

• Require no pretreatments

• Microdissect fresh frozen, sucrose treated and live tissue

• Isolate high quality RNA, DNA and protein

• Collect individual cells

• For downstream single cell analysis, such as NGS, transcriptome sequencing and Omics applications

Cell isolation unit (CIU) is a vacuum-assisted single cell collection and tissue microdissection instrument based on capillary-based cell and tissue acquisition system (CTAS; USPT 8,797,644; Kudo et al, 2012) that can acquire individual cells from cell cultures and tissues. Our patented vacuum impulse assisted collection permits rapid acquisition of single cells from any untreated cell cultures without affecting their viability, providing reliable and affordable means to perform various downstream experimentation. Single cell in as little as 15 nl volume may be collected for further whole genome analysis or clonal expansion. Cell cultures require neither special treatment nor specific peripherals, thus enabling unmatched flexibility in experimental design and sample collection. Moreover, the instrument is suitable for individual cell collection and region specific acquisition of subanatomical areas from complex heterogeneous tissues such as the brain. Operation of the system is intuitive and requires minimal training. Cell cultures require no treatment or specific peripherals, thus enabling unmatched flexibility in experimental design and sample collection.

 

Even RNA isolated from collected cells and tissue regions exhibits high integrity and low degradation rates with RIN >9.0. Subsequent labeling protocols (e.g. T7 based) consistently produce high incorporation yields making it suitable for downstream PCR, microarray and sequencing analyses.

 

The instruments’ low cost, ease of use, single cell resolution, and minimal impact on cell viability, make it unique and vital research tools. Importantly, it is a free standing instrument that can be mounted over a wide range of inverted microscope models, providing additional flexibility in the laboratory. Operation of the system is intuitive and requires minimal training.

 

Perhaps one of the most distinct features is the ability of CIU to collect live cells for further

reculturing and a plethora of downstream functional studies. Both individual fluorescently labeled and morphologically distinct cells can be acquired from any cell cultures or tissue sections.

 

In summary its notable features include:

- Simple operation with minimal training

- Single cell collection from untreated cultures and tissue sections

- Efficient tissue microdissection

- High viability of collected cells

- High quality RNA and protein for downstream –omics studies

- Retract Function – easier to work with multiwell plates

- Recalibration Function – no recalibration between samples

- Side Swivel Head – ergonomic design for handling DCUs

- Flexibility and versatility - works with most inverted microscopes such as your existing microscope

- Fraction of the cost of any laser assisted microdissection instrument


Specification:

-Resolution of isoltaion: Single Cell

-Recalibration Function: Yes

-Power Supply: Input 100-240 V, 50-60 Hz, 1.5 A; Output + 24 V, 2.7 A

-Illumination: Light 144 LEDs ring light, Input 24V DC

-Vacuum range: up to 22”Hg

-Ready-to-use sterile DISPOSABLE CAPILLARY UNIT (DCU): travel step 1.5 μm, max. travel: 23.3 mm

-Available DCU IDs, μm From 10 to100 μm, most common DCUs: ID=20 μm;  ID=30 μm; ID=50 μm;  ID=70 μm all in 6 units/box

-Specimen holder: 25 mm slides, 35 mm Petri dish and multiwell plates 6-, 12-, 24- 6-, 12-, 24-, 48-, 96-

-Vacuum duration (Ts), seconds: 0.1 to 1.0 s

-Vacuum strength, -mm Hg: 111.8 to 558.8 Hg

-Acquisition speed (-mm Hg/Ts), seconds

-Minimum settings: (111.8/0.1 sec) 1.3 s

-Maximum settings: (558.8/1.0 sec) 2.2 s

-Acquisition sample volume (from Lowest to Highest settings): DCU20 (μm) 10 nl to 2.5 μl, DCU30 (μm) 35 nl to 3.0 μl, DCU40 (μm) 70 nl to 5.0 μl

-Cell collection speed (cells/minute) from tissue sections: Rat Purkinje cells (cerebellum) 12.0 ± 1.5 c/min, Mouse anterior horn motor neurons 12.0 ± 1.5 c/min

-Cell collection speed (cells/minute) from adherent cell cultures: SH-SY5Y human neuroblastoma cell line Up to 25 c/min, Chinese hamster ovary cells (CHO) Up to 25 c/min

 

Cell isolation unit plus (CIU+) is a universal platform for tissue microdissection, single cell acquisition, and deposition into the single wells for further molecular analysis or clonal expansion. The main working principle is based on the capillary based vacuum pulse assisted technology (CTAS; Kudo et al, 2012). Developed system can microdissect tissue sections, collect and deposit individual cells from any adherent cultures grown in standard cell culture dishes in as small as 15 nl volume, compatible with various downstream single cell analyses and next generation sequencing. Like CIU, the system may be used with a wide range of inverted microscopes. The benefits include cost-efficiency, simple operation, digital interface, complete workflow from single cell isolation to downstream analysis, compatibility with a wide range of inverted microscopes and use of standard plates and culture dishes.

CIU+ features:

-Compatibility with a wide range of inverted microscopes

-Full digital control with functions equivalent to CIU

-One button intuitive calibration

-Collection and deposition of up to 48 samples

-UNIVERSAL STAGE ADAPTER: allows for collection and deposition of samples in multiple configurations - from slide to slide, plate to plate, slide to plate, slide or plate to PCR tubes, 8-tube strips or a 48-well plate

Specification:

-Interface: Digital

-Max Pressure: 5 PSI

-Max Vacuum: 10 PSI

-Impulse Duration 0.1 to 1.0 sec

-Sample compatibility: Adherent cells, suspended cells, 3D cultures, native, fresh frozen,

sucrose treated and fixed tissues

-Resolution: Single cell for cultures and 20 μm ID for tissue sections

-Viability of Collected Cells: up to 95%*

-Available DCU IDs: 10 to 100 μm, Travel Step 1.5 μm

-Specimen Holder: 25 mm slides, 35 mm and 50 mm Petri dishes, 48 PCR tube holder

-Deposition Capacity: up to 48 samples

-Acquisition Volume: DCU20 μm - 10 nl to 2.5 μl, DCU30 μm - 35 nl to 3.0 μl, DCU40 μm - 70 nl to 5.0 μl

-Collection Speed Tissue sections - up to 12 cells/minute, Cell culures - up to 25 cells/minute

-Microscope compatibility a wide range of inverted microscopes

 

previous item: Installation & operation next item: TIRF Microscopy