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Femtonics Peripherials

Femtonics Peripherials

Detail Description

Choices of microscope:

FemtoSmart is the first member of Femtonics next-generation two-photon microscope series. In the design the main goal was to create a totally customizable microscope for in vivo studies with a distinct, charismatic appearance. The special feature of this microscope is the elevated body, which allows extremely free positioning of the sample below the objective.

The column-based X-Y-Z moving body houses the scanner unit, control circuits and internal light path. The footprint of the leg is only 250 mm x 250 mm and there is a large space below the objective. The design enables the objective to move in a 50 mm range in Z direction and the microscope can move in an XY direction around the base in 50 mm range. The tilting objective can further increase mobility. The large space under the objective, where you can place virtual reality, movable tables, electrophysiological devices, is extremely useful for behavioral studies and supports functional studies of model organisms from C. elegans to non-human primates.


Femtonics tilting objective:

Tilting objective driven by two motors to rotate the objective around horizontal and vertical axes gives a higher level of freedom in positioning the objectivefor in intravital imaging, deep brain imaging in rodents or even non human primates.

Novel surgical methods utilizing grin lenses or miniature prisms allow optical investigation of deep structures within the brain. Implementing these technologies is greatly enhanced by our high-precision motorized tilting objective:

•aligning optical axis of the embedded optical grin lens is facilitated by the handwheel operated multi-axis positioning of the tilting objective
•store and reuse optimal angles and positions when experimenting multiple times with the same animal

SPECIFICATION

•rotation around horizontal X axis: ±90°
•rotation around vertical Y axis: ±50°
•movement with piezo: up to 400 µm (recommended)
•speed of rotation: 20°/sec
•maximum number of mounted detectors: 3
•unidirectional repeatability < 0.02 mrad
•bidirectional repeatability < 1 mrad

BENEFITS

•flexible objective positioning with high precision
•motorized, software stoarge of the position
•backlash-free, high stability at all positions
•minimal dispersion, no optical aberration
•minimized optical light path, detectors equipped to tilting unit
•optional: fast movement of the objective in Z-direction with Piezo objective positioner
•optional green lamp illumination

FemtoStab: Beam stabilization unit for sharing MP laser between 2 microscopes

High precision two-photon measurements or more complicated optics in the beam path require more precise automatic beam stabilization. FemtoStab is a precise, automatic beam alignment system, which compensates for the slow and fast time scale laser beam misalignments caused, e.g. by thermal changes & the warm-up procedure of the laser or air movements, and mechanical vibrations.

This fast system is capable of a several hundred Hz refresh rate, it is therefore very fast and precise to compensate for small angle vibrations of the tables in real time. It is a standalone system and needs no computer control but can be connected to a PC to show its graphical user interface.

Equipment for sharing laser sources between two or more independent optical tables. This equipment distributes light from one laser source to more than one setup which can be situated even on mechanically independent optical tables. Therefore, measurements sensitive to mechanical vibrations can be performed on two or more tables at the same time. As setups are optically but not mechanically connected, individual imaging experiments can be performed in each setup without mechanically perturbing the experiments in the other setups. This also allows independent micromanipulation such as patch-clamping in each setup without risking the measurement on the other setup.

Advantages:

•Divide imaging laser light between two setups on the same optical table

•Quickly switch uncaging / photo-stimulation light between setups

•Couple laser beams to other mechanically independent optical tables


Femto holographic photostimulation unit

The holographic stimulation is an extremely flexible device producing simultaneous illumination in variable shape and size of multiple regions in the three-dimensional sample for optogenetics, uncaging and other methods that are based on simultaneous activation of various regions. The simultaneous illumination of different regions is established by diffractive Spatial Light Modulator (SLM). The SLM consists of a parallel-aligned nematic liquid crystal layer to disperse and to shape the incoming laser beam and to change the phase of light. This converted holographic beam excites multiple spots with arbitrary shapes in pre-determined depths simultaneously.  The software calculates the corresponding phase-hologram and addresses the pattern to the crystal layer.


Noise dampening box

We provide sound dampening boxes for power supply of MaiTai lasers. The noise levels are reducced to than of an ordeinary PC. And these boxes can be easily fitted under standard optical tables.This box was designed specifically for the power supply and chiller units of SpectraPhysics MaiTai lasers. Custom designs for other lasers or lab instruments are available, too.


Shading box

This special shading box enables high sensitivity external detection of the fluorescent photons even in a daylight room and serves as a Faraday cage and dust cover as well.

Standard box:

•1m x 1m x 1m size
•Roller curtain
•Three cable entry tubes

Other shading boxes options:

•Customized sizes
•Custom cable openings
•Acoustic noise isolation
•Internal lights

Different doors:

•Roller curtain
•Telescopic tilting door

Motorized Stages

Sutter Stage MP-78

•Baseplate: 200x375x55mm
•Max. travel: 25mm
•Resolution: 0.2 – 0.04 µm/step
•Max. Load: 50kg
•Weight: 16kg
•RS232 port
•Motorized legs
•Contoller
•Handwheel cube
•Suitable for in vivo and in vitro measurements
Luigs & Neumann
•Shifting table 380FM-U
•Bridge 500 (in vitro)
•In Vivo Bridge
•Easy exchange system
•Shaped headholder
•Keypad & Control Box


MicroManipulators

Sensapex Micromanipulatior
•Compact-size
•Superior stability
•Piezo-drives
•Battery operated control unit
•3-axis free movement
•Handwheel box
•Software controlled
•Six speed settings
•Mount: bolt or magnet
•Flip or rotation mechanism

Luigs & Neumann Micromanipulatior

•Traverse path X / Y / Z : 25mm, motorized / 28mm, manual
•Motor resolution : 9,8nm •Reproducibility :<1µm
•Weight : 2,2 kg
•Spindle lead : 0,5mm per rotation
•Software controlled 
•3-axis free movement
•Flip or rotation mechanism


Animal head holder and heating pad

•Head holder for mice/rat with three magnetic holders
•Simplified stereotaxic frame adaptor
•Heated pad to the head holder
•Biological temperature controller

This clamping method is based on 3 point fixation (mouth, nose and ear) that makes the stable fixation. The nosepiece can be detachable and changeable by using the dedicated anesthetic mask, and it will be easily moved onto the experiment with use of anesthesia. By attaching the holding instrument to a single bar, it is enable to fix the head stably and allows the head angle to be freely adjusted through 360°. The shape and functions are simple, yet this holder can be used for many different purposes. The SGM-3 is for rats. The SGM-4 is for mice. The SG-3N (for rats) and SG-4N (for mice) without the accessories of anesthetic mask and tilt adjustment mechanism are also available.

This clamping method is based on three-point fixation (mouth, nose and ears), ensuring that the head is easily and firmly fixed in position. By attaching the mouth/nose clamp and ear bar to a single bar, the instrument allows the head angle to be freely adjusted through 360°. The holder's overall compact size makes it possible to be used for many different purposes. It can be placed on the iron plate in combination with a thin type magnetic stand (GJ-12). The SG-3N is for rats, and the SG-4N for mice.

This head holder comes with a holder and a base of 100% plastic and is used in MRI. The animal's head can be firmly held in place at three-points (mouth, nose and ears). The size of the SGP-3 for rats is kept to just under 70mm when the holder is detached from the base. If an MRI scanner has a small opening, the animal can be held in place to pass through the scanner. This allows measurements even when using small MRI scanners. The SGP-3 is for rats and the SGP-4 is for mice.


Dual Perfusion Chamber Kit

1) Recording chamber kit with mobile polypropylene mesh for slice holding

2) Dual-channel In-line Solution Heater

3) Bad temperature controller

4) Dual channel bubble trap

5) Vent syringes - 2 for the bubble trap and 2 for the double chamber

6) At least 1mm diameter teflon tubes for perfusion – narrower diameter for the dual inflow perfusion and wider diameter tubes for single outflow perfusion. Short pieces of silicon tubes for the connection of teflon tubes, dual-superfusion chamber, bubble trap and In-line heater head

7) Stainless steel tube with bevel opening in the perfusion outlet sucktion essential for the regulation of the flow level and stability

8) Harp slice grid put it on the tissue to eliminate movements

9) Ground-connection (for elctrophysiology) is necessary to the online-heater and/or to the bubble trap

 

Virtual Reality

The Jet Ball is a novel virtual reality experimental system. It is based on an air supported spherical treadmill, and allows a restrained animal to navigate in virtual space. Virtual reality provides a perfectly controllable experimental environment, and can be used for investigating navigation, cognition, learning and memory.

Applications

•Virtual reality navigation
•Electrophysiological and optogenetic methods
•2-photon laser scanning microscopy
•Intracellular dynamics during virtual navigation •
Operant conditioning
•Learning and memory processes

Key advantages

•Restrained animal performing voluntary behaviour
•Easy interfacing with electrophysiology setups
•Retractable operant device for reinforcement: positive (water) and negative (air puff)
•Common mazes or customized virtual reality scenes
•Operant conditioning protocols
Options
•Third party custom micromanipulators available
•Sensory stimulation: 3D sound system, fast-response odour generator, tactile stimulator
•200 mm ball for mice, 300 mm ball for rats
•Custom programmed operant conditioning protocols and VR scenes
•Base with motor-driven XY linear table for large area brain scanning with 2-photon laser

Intrinsic Imaging System

The system is optimized for non-invasively localizing the areas of the brain or the spinal cord which are activated during different stimuli or combinations of stimuli. This product serves as a monitoring tool before or during in vivo experiments. It can be installed as a stand-alone system or can be integrated into any Femtonics microscope.

Based on:

•Measurement of reflection change of the cortical surface
•The source of the reflection change is either the change in oxygenization of the blood (if measured at 595nm), or change in blood volume (550nm)

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