OptoFlash: LED illumination for flash photolysis & Optogenetics
The developments for the Optoflash application are basically twofold. First, although the Optoflash can be set for continuous illumination if required, it
incorporates a front panel controlled timer, which generates flashes anywhere from 50 microseconds to 100 milliseconds, via either a front panel switch or an external trigger input. Alternatively, flashes of arbitrary
duration can be generated, either under manual or external control. An optical monitor output is also provided, and just as for proven OptoLED design, it can optionally be used to drive a feedback system to guarantee a constant optical output during a pulse.
The second development is a coupling system for coupling our standard LED head design into a range of optical fibres, of diameter from 0.1 to 1mm. For fibres less than 1mm diameter, simultaneous illumination of multiple fibres by a single LED is supported. A high efficiency optical relay system is used to focus light from the LED onto the fibre entrance.
Our LED protection networks safely allow a certain degree (depending on the LED) of over-driving during pulsed illumination such as that during a flash. The optical relay system also has provision for inclusion of filtering to limit the bandwidth of the LED if required.
•Flash duration controllable from 50usec -100msec by internal timer
•Durations of arbitrary length via external control
•Flash rise and fall times less than 0.1 μsec
•Optical monitor output, with optional optical feedback
•Coupling system for optical fibres up to 1mm diameter for in-vivo optogenetic stimulation
•>30mW/mm² using a 200μm fibre when continuously driven by 470nm LED
•Protection network allows at least 2x over-driving for short pulses
OptoLED head - 365nm
OptoLED head - 385nm
OptoLED head - 405nm
OptoLED head - 455nm
OptoLED head - 470nm
OptoLED head - 490nm
OptoLED head - 505nm
Fibre ends can in principle be finished in a variety of ways - please ask for details. And of course the Optoflash can be coupled into any microscope, using exactly the same accessories as for the OptoLED.
OptoLED head - 530nm
OptoLED head - 565nm
OptoLED head - 590nm
OptoLED head - 627nm
OptoLED head - 660nm
OptoLED head - 740nm
OptoLED head - 850nm
OptoLED head - 940nm