32/35mm cover-glass botto/perfusion cell dish：use in oil immersion objective for high quality DIC & fluorescent imaging; minimize heat sink phenomena
In an open dish it will take 6-10 complete volume changes to get a 90-95% concentration change. So if the open dish contains 3-5ml of media it will have to wash with 18 to 50ml of media where there is no profiled flow over the cell viewing region. If you are looking to do a complete wash it is best to use a closed chamber, parallel plate flow chamber. With a flow chamber under closed perfusion the media is a nearly even exchange so any media in the optical cavity will be forced through the chamber and not mixed into the existing media then removed. In changing perfusate sources under a closed chamber the fluid is completely flushed very rapidly. Our PFC closed perfusion volume is around 200uL with the optical window thickness of the laminar flow path around 1.4 mm. Uses 0.4mm ID/1.8mm OD silicone tubing for connection.