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Glass bottom/perfusion cell dish

Glass bottom/perfusion cell dish

Detail Description

32/35mm cover-glass botto/perfusion cell dish:use in oil immersion objective for high quality DIC & fluorescent imaging; minimize heat sink phenomena

In an open dish it will take 6-10 complete volume changes to get a 90-95% concentration change. So if the open dish contains 3-5ml of media it will have to wash with 18 to 50ml of media where there is no profiled flow over the cell viewing region. If you are looking to do a complete wash it is best to use a closed chamber, parallel plate flow chamber. With a flow chamber under closed perfusion the media is a nearly even exchange so any media in the optical cavity will be forced through the chamber and not mixed into the existing media then removed. In changing perfusate sources under a closed chamber the fluid is completely flushed very rapidly. Our PFC closed perfusion volume is around 200uL with the optical window thickness of the laminar flow path around 1.4 mm. Uses 0.4mm ID/1.8mm OD silicone tubing for connection.

• Its best to have the pump media and chamber all at the same horizontal level to keep gravity from effecting your chamber and not creating a water column.
• For high flow rates it may be required to pre-warm your media before it enters the chamber to prevent cell shock.
• When removing media from the chamber you also want to make sure not to create a water column, this can be achieved by running a section of tubing off to the edge of the stage, cutting the tubing then placing the tubing into a vertical larger tube.
• You also want to prevent droplets from forming on the tubing which can cause a pressure difference in the tubing coming from the chamber (vacuum) causing the coverslip to flex. This can be achieved by using string or cotton and threading it into the tubing that you cut at the edge of your stage. This allows the media to slowly wick out onto the string then down the larger tube not causing the water column.
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